Curriculum Vitae

Cum Laude, Departmental Honors, and Dean's List

Cum Laude and Dean's List

• Successfully conducted in-depth analysis and visualization of extensive experimental data collected during previous dissertation research, laying the foundation for forthcoming publications.

• Completed dissertation: Sex Differences and Neuroimmune Effects of Microglial Response in the Mesolimbic Reward Pathway in Nicotine Substance Use Disorder
• Designed and executed comprehensive study investigating neuroimmune modulation and microglial response in male and female mice in nicotine use disorder funded via NIDA/NIH R36 grant.
• Demonstrated independence by successfully managing multiple tasks, priorities, and deadlines for a dissertation project following the relocation of my supervisor to another university mid-project.
• Communicated effectively both in written and oral forms, producing comprehensive reports and peer-reviewed publications and delivering engaging presentations to convey complex research findings to diverse audiences.
• Supervised and provided guidance to professional and undergraduate students in the execution of independent research projects, fostering their development and contributing to the growth of the research program.

• Assay design and development to screen therapeutics for TP53 synthetic lethality in standard cell lines and patient derived organoids.
• Performed methylation analysis of Carolina Women's Care Study clinical samples (longitudinal set) including experimental design and validation of procedures, bisulfite conversion, whole genome amplification, and pyrosequencing.
• Validated and optimize fluorescence based in-vitro assays to study potential anticancer compounds actively targeting the cell cycle.
• Developed Y2H methodology for disruption of protein-protein complexes and identify lead targets for DNA damaging chemotherapeutic agents.

• Created bacterial genomic Next Generation Sequencing libraries using tagmentation of index adapters.
• Executed small scale characterization of Cry genes including electroporation of shuttle vector plasmids into acrystalliferous Bacillus thuringiensis, phase contrast microscopy for crystal formation, whole culture and crude protein extraction for protein analysis and bioassays for activity.
• Prepared electrocompetent B. thuringiensis (Cry- AB227 HD73-102 strain) cells.

• Developed and performed in-vitro functional assays, characterize secondary leads, and provide support for high throughput screening (HTS), including data review, QC, and hit selection for inhibitors of androgen and estrogen receptor function; techniques included RT-PCR, transactivation assays, cellular proliferation assays, and M2H dual luciferase assays.
• Characterize effects of small molecules or kinase inhibitors on protein expression, gene expression, cell activation, viability and proliferation (includes protein degradation screening by In-Cell Western).
• Assisted with assay development for calcium-induced aequorin in Arabidopsis thaliana (96-well format).
• Contributed to the analysis of profiling and small molecule library screening data.

• Characterized effect of small molecules on nuclear receptors in primary screen; pursue follow-up characterization of optimized leads via secondary cell-based assays.
• Performed conformation profile screening using cDNA and T7 phage display.

• Conducted cloning, assay development, and protein production and purification for proprietary compounds to be used in neurological and anti-inflammatory research.

• Established methods and protocols for miniaturization and industrialization of nuclear receptor profiling assay in shared small molecule screening facility.
• Implemented medium throughput screening via mammalian two-hybrid for development of a nuclear receptor conformational profiling tool/platform.

• Developed assays for peptide libraries and IFBM coating technology on synthetic and biological surfaces.

• Worked in drug discovery process to bring candidates into clinical trials.
• Conducted assay development for high throughput M13 phage display screening and ELISA screening.
• Performed HTS data reduction and analyze and manage large data sets and databases.

Keady, J., Fisher, M., Anderson, E., LeMalefant, R., Turner, J. (2022). Age-specific impacts of nicotine and withdrawal on hippocampal neuregulin signalling. European Journal of Neuroscience, 2022 Sep;56(6):4705-4719.  https://pubmed.ncbi.nlm.nih.gov/35899607/

Kumar, M., Adeluyi, A., Anderson, E.L., Turner, J.R., 2020. Glial cells as therapeutic targets for smoking cessation. Neuropharmacology 108157. https://doi.org/10.1016/j.neuropharm.2020.108157

O’Donovan, B., Adeluyi, A., Anderson, E. L., Cole, R. D., Turner, J. R., Ortinski, P. I. (2019). Altered Gating of Kv1.4 in the Nucleus Accumbens Suppresses Motivation for Reward. eLife, 8, e47870. https://doi.org/10.7554/eLife.47870.001.

Anderson, E.L. , Banister, C.E., Kassler, S., Messersmith, A., Pirisi, L., Creek, K.E., Wyatt, M.D. (2016). Human Papillomavirus Type 16 L2 DNA Methylation in Exfoliated Cervical Cells from College-Age Women. Journal of Lower Genital Tract Disease, 20(4), 332-7. http://doi.org/10.1097/LGT.0000000000000251.

Premnath, P. N., Craig, S. N., Liu, S., Anderson, E. L., Grigoroudis, A. I., Kontopidis, G.,  McInnes, C. (2015). Iterative Conversion of Cyclin Binding Groove Peptides into Druglike CDK Inhibitors with Antitumor Activity. Journal of Medicinal Chemistry, 58(1), 433–442. http://doi.org/10.1021/jm5015023.

Premnath, P. N., Liu, S., Perkins, T., Abbott, J., Anderson, E., & McInnes, C. (2014). Fragment Based Discovery of Arginine Isosteres Through REPLACE: Towards Non-ATP Competitive CDK Inhibitors. Bioorganic & Medicinal Chemistry, 22(1), 616–622. http://doi.org/10.1016/j.bmc.2013.10.039.

Norris, J. D., Joseph, J. D., Sherk, A. B., Juzumiene, D., Turnbull, P. S., Rafferty, S. W., Cui, H., Anderson, E., Fan, D., Deng, X., Kazmin, D., Chang, C.Y., Wilson, T.M., McDonnell, D. P. (2009). Differential Presentation of Protein Interaction Surfaces on the Androgen Receptor Defines the Pharmacological Actions of Bound Ligands. Chemistry & Biology, 16(4), 452–460. http://doi.org/10.1016/j.chembiol.2009.01.016.

Ashraf, S. S., Anderson, E., Duke, K., Hamilton, P. T., & Fredericks, Z. (2003). Identification and Characterization of Peptide Probes Directed Against PKCα Conformations. The Journal of Peptide Research, 61(5), 263–273. http://doi.org/10.1034/j.1399-3011.2003.00056.x.

Anderson, E, Hessing-Alpers, M, Keady, J, Turner, J (2020), Sex Differences in Mesocorticolimbic Reward Pathway Microglial Response in Nicotine Substance Use Disorder. International Behavioral Neuroscience Society 2020 Podium Presentation. Glasgow, Scotland, United Kingdom (Canceled Covid-19).

Anderson, E.L., Adeluyi, A., Turner, J.R. (2019). Neuroinflammatory Modulation of Nicotine Dependence. Society for Research on Nicotine & Tobacco 25th Annual Meeting, Podium Presentation, San Francisco, California, USA.

Anderson, E.L., Adeluyi, A., & Turner, J.R. (2018). Neuroinflammatory Modulation of Nicotine Dependence. International Behavioral Neuroscience Society 2018 Annual Meeting. Boca Raton, FL, USA.

Anderson, E.L., Twiss, J.L., Turner, J.R. (2017). Fluorescent Imaging: A Neurotechniques Journey. Instrumentation Resource Facility Research Symposium, Columbia, SC, USA.

Anderson, E.L., Twiss, J.L., Turner, J.R. (2017). Fluorescent Imaging: A Neurotechniques Journey. University of South Carolina 2017 Neuroscience Retreat, Columbia, SC, USA.

Anderson, E.L., Banister, C.B., Liu, C., Buckhaults, P.B. (2015). Screening Therapeutics for TP53 Synthetic Lethality. Center for Colon Cancer Research 2016 Annual Retreat, Columbia, SC, USA.

Anderson, E.L., Banister, C.B., Kassler, S., Messersmith, A., Pirisi-Creek, L., Creek, K.E., Wyatt, M.D. (2015). CpG Methylation of HPV16 L2 In a Cohort of College-age Women. Carolina Women's Health Research Forum, Columbia, SC, USA.

Anderson, E.L., Banister, C.B., Kassler, S., Messersmith, A., Pirisi-Creek, L., Creek, K.E., Wyatt, M.D. (2015). pG Methylation of HPV16 L2 In a Cohort of College-age Women. The 30th International Papillomavirus Conference (HPV 2015), Lisbon, Portugal.

Premnath, P. N., Craig, S. N., Liu, S., Anderson, E. L., Grigoroudis, A. I., Kontopidis, G.,  Perkins, T., Wyatt, M.D., Pittman, D.L.,McInnes, C. (2014). Conversion of Peptidic Inhibitors of Cyclin Binding Groove Derived From p21 and p107 to Drug Like CDK Inhibitors Utilizing REPLACE Strategy. Conference: 3rd Protein kinase Conference, Boston, MA, USA.

Christensen, D., Paige, L., Buehrer, B., Antonio, B., Anderson, E., Kang, J., Barnett, T., Hamilton, P., Fowlkes, D.M., (1999). Molecular Braille: A Novel Technology for Identification and Characterization of Compounds that Modulate Receptor Function. The 5th Annual Society for Biomolecular Screening Conference and Exhibition, Edinburgh, UK.

Covering: Drug Development and FDA meetings, applicability of FDA drug regulations to Clinical Studies, IND content, format, review and maintenance,  medical device development and FDA meetings,  applicability of FDA Device Regulations to Clinical Studies, IDE preparation, submission, and maintenance.

Identification of a good drug discovery target, assay construction, elements of medicinal chemistry,  animal efficacy testing, the principals of ADME studies and safety/toxicology testing, and the steps required to prepare an IND document.

Overview of multidisciplinary, foundational concepts that underlie the genetics of addiction.

• Recognized for work with the Delta Alpha Pi International Honor Society for students with disabilities

• Led laboratory sessions for 47 students (20 hours/week).

• Led laboratory sessions for 46 students (20 hours/week).